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6×DNA Loading Buffer
Laemmli buffer Preparation 1x2x 4x and principle
Native Gel
Cold Spring Harbor Protocolshttps://cshprotocols.cshlp.org/content/2013/10/pdb.rec078733.full?text_only=trueNative Gel-Loading Buffer - CSH ProtocolsReagent Quantity (for 10 mL) Final concentration; TBE (10×) 2.5 mL: 2.5×: Glycerol (100%) 5 mL 50%: Bromophenol blue (1%) 1 mL 0.1%: H 2 O : 1.5 mL: Take care to be accurate when measuring viscous solutions such as glycerol.
6×SuperStain Loading Buffer
Laemmli Loading Buffer 5× pH 68
Cold Spring Harbor Protocolshttps://cshprotocols.cshlp.org/content/2016/3/pdb.rec088336.full?text_only=trueLaemmli Loading Buffer (5×, pH 6.8) - CSH ProtocolsLaemmli Loading Buffer (5×, pH 6.8) 2.5 mL 2 m Tris-HCL (pH 6.8) . 2 g sodium dodecyl sulfate. 100 mg bromophenol blue. 10 mL glycerol. 1.542 g DTT. Add up to 20 mL ...